Lomov, Yu.M., Agafonova, V.V., Telesmanich, N.R., Terentyev, A.N., Karbyshev, G.L., Kochetkova, A.P., Akulova, M.V.

Detection of lipase in Vibrio cholerae serogroup O1 in reaction of volumetric agglomerationt

Research Institute for Plague Control, Rostov-on-Don, Russia

Study showed that El-Tor strains of V.cholerae isolated from different sources produce lipase for hemolysis after cultivation during 24 h on meat-peptone broth independently from their toxigenic and hemolytic abilities. Study of 3- and 4-hours broth cultures of vibrios revealed possibility to differentiate between hemolytic nontoxigenic strains and toxigenic nonhemolytic ones. Using antilipaze diagnostic kit it was possible to differentiate El-Tor vibrios from vibrios of classic biovar basing on lipase production 24 h after cultivation on meat-peptone broth that was evident in El-Tor vibrios but not in classic biovar strains.
Zh. Mikrobiol. (Moscow), 2007, No. 1, P. 74—77