Kovalchuk, L.V., Khoreva, M.V., Varivoda, A.S., Pashchenko, O.E., Gracheva, L.A., Bykova, L.P., Kondratenko, I.V., Bologov, A.A.

Effect of Toll-like receptors (TLR) ligands on in vitro synthesis of proinflammatory cytokines by peripheral blood mononuclear cells from healthy men and from patients with common variable immunodeficiency

Russian State Medical Institute, Moscow, Russia

Production of citokines of tumor-necrosis factor (TNF)-α and interferon (IFN)-α by peripheral blood mononuclear cells (PBMC) from healthy men and from patients with common variable immunodeficiency (CVID) after stimulation with zymosan, lypopolysaccharides, flagellin and CpG, which are ligands of TLR 2/6, TLR 4, TLR 5, TLR 9 respectively, was studied in vitro. In healthy men production of TNF-α varied between individuals, whereas synthesis of IFN-α was similar. Spontaneous production of TNF-α by PBMC in patients with CVID was increased and accompanied by decrease in TNF-α production stimulated by each analyzed ligands except CpG. Observed changes in TLR-dependent TNF-α production can play important role in pathogenesis of CVID.
Zh. Mikrobiol. (Moscow), 2007, No. 1, P. 38—42