Institute of Immunology, Moscow, Russia; National Heart and Lung Institute; Wright Fleming Institute of Infection and Immunity, Imperial College, London, UK
Role of several types of cells (human broncho-epithelial cells, BEAS-2B cell line, and mononuclear cells as model of macrophages) in production of α-, β- and γ-interferons during acute respiratory viral infection was studied. Kits for detection of these interferons by quantitative PCR assay has been developed. In human broncho-epithelial cells respiratory viruses induced statistically significant expression of α-interferon mRNA at 8 hours after infection, β-interferon mRNA — at 24 hours after infection, IL-29 mRNA (γ-interferon) — at 24 hours after infection, IL-28 mRNA (γ-interferon) — at 8 and 24 hours after infection. In BEAS-2B cell line induction of α-interferon mRNA expression was observed at 8 hours after infection, β-interferon mRNA expression — at 24 hours after infection, IL-29 mRNA (γ-interferon) expression — at 8 and 24 hours after viral challenge. Production of β- and γ-interferons by ELISA at 24 hours after infection has been detected. When polymorphonuclear cells were challenged, induction of α-, β-, and γ-interferons expression was observed at 8 hours after infection. Production of α-, β- and γ-interferons has been detected by ELISA at 24 hours after infection by rhinovirus 16.
Zh. Mikrobiol. (Moscow), 2006, No. 7, P. 63—69