Balabanov, D.N., Rakovskaya, I.V., Gorina, L.G., Goncharova, S.A., Gamova, N.A., Barchatova, O.I.

The comparison of Mycoplasma detection methods in the urogenital tract infection

Gamaleya Research Institute of Epidemiology and Microbiology, Pediatrics clinic of Medical Academy, Моscow, Russia

Six different methods have been employed to detect M.hominis (Mh) and U.urealyticum (Uu) in clinical samples collected from 67 men. The results obtained by PCR and IF test were approximately equal: 13.6 and 13.44% — Mh and 44.4 and 48.8% — Uu, respectively. Mycoplasmas were detected by cultural method less frequently (9.6% — Mh, 32.2% — Uu). The highest infection rates were obtained in the test for blood antigens (40% — Mh and 63% — Uu). At present a commercial diagnosticum to detect mycoplasma antigents in blood is lacking. Sometimes the results of cultural method are positive, while the PCR results are negative. So the optimal scheme based on both PCR anf culture has been proposed.
Zh. Mikrobiol. (Moscow), 2006, No. 4, P. 82—85