Shmarov, M.M., Tutykhina, I.L., Logunov, D.Yu., Verkhovskaya, L.V., Nedosekov, V.V., Cybanov, S.Zh., Novikov, B.V., Naroditsky, B.S., Gintsburg, A.L.

The induction of protective immune response in mice vaccinated by recombinant avian adenovirus CELO expressing glycoprotein G of the rabies virus

Gamaleya Research Institute of Epidemiology and Microbiology, Moscow; Research Institute of Veterinary Virology and Microbiology, Pokrov, Russia

The recombinant avian adenovirus CELO-gpRb expressing glycoprotein G of rabies virus (strain TS-80, ARRIVV&M, Pokrov, Russia) was used for mice vaccination against rabies. Double intramuscular immunization by recombinant CELO-gpRb adenovirus in a dose 10⁹ pfu per mouse caused the induction of virus neutralizing antibodies (VNA) synthesis in 78% of mice, while twice repeated intradermal injections of the recombinant adenovirus failed to induce the VNA production. The protection level in groups of vaccinated mice after intracerebral injection of CVS rabies virus in a dose of 100 MLD50 was equal to 45% at single intramuscular immunization and to 91% after twice repeated intramuscular immunization. The recombinant adenoviral vaccine against rabies, based on CELO viral genome, has a good perspective for domestic and wild animal vaccination, not only due to rather high protection level, but also because the production of adenoviral CELO vaccine in chicken embryos is of high technology and inexpensive.
Zh. Mikrobiol. (Moscow), 2006, No. 4, P. 69—71