Research Institute of Epidemiology and Microbiology, Vladivostok, Russia
The one of general pathogenic characteristic of Yersinia pseudotuberculosis is the ability to intraphagosoma surviving in neutrophils. The enzymic activity of neutrophils in pseudotuberculosis infection by in vivo and in vitro models was studied. The simultaneous increase in the activities of the enzymes participating in the active oxygen intermediates production in Y.pseudotuberculosis infected neutrophils was determined. The increase of myeloperoxidase activity in initial terms of infection reflected the neutrophil defensive reaction to the inoculation of bacteria. The following reduction of cytochromoxidase and myeloperoxidase activities in infected phagocytes determined the damaging effect both Y.pseudotuberculosis and active oxygen intermediates generated by the cells during phagocytosis. The obtained data on intracellular contents of oxide metabolites in infected neutrophils indicated the associating processes of the nitric oxide and active oxygen intermediates generation. Herewith, it is necessary to note the presence of the similar processes in Y.pseudotuberculosis infected neutrophils both on the models in vitro and in vivo (the inflammation focus phagocytes).
Zh. Mikrobiol. (Moscow), 2006, No. 3, Supplement, P. 43—47