Donin, M.V., Boichenko, M.N., Vorobyev, A.A.

Evaluation of the persistence a Salmonella enteritidis recombinant strain with HBc antigen expressed in the body of mice and macrophage culture

Sechenov Medical Academy, Moscow, Russia

The behavior of S.enteritidis recombinant strain E-23/pKDNA_3.1 with HBc antigen expressed by the eukaryotic promoter (DNA vaccine) in the body of mice after their infection per rectum and in the culture of macrophages was studied. The expression of HBc was shown to lead to the loss of the capacity of salmonellae for persistence in parenteral lymphoid tissue and for inducing the formation of anti-HBc antibodies. The study of the interaction of salmonellae with the macrophage culture revealed that the synthesis of HBc antigen inhibited the intracellular multiplication of salmonellae.
Zh. Mikrobiol. (Moscow), 2006, No. 3, P. 45—48nKey words: salmonellae, HBc antigen, DNA vaccine, macrophages