Zadnova, S.P., Isaev, N.D., Kuteikin-Teplyakov, K.B., Tikhonova, O.V., Toropygin, I.Yu., Archakov, A.I., Smirnova, N.I.

Proteomic analysis of two isogenic Vibrio cholerae of the classical biovar with the alternative expression of virulence genes

Research Institute for Plague Control “Microbe”, Saratov; Research Institute of Biomedical Chemistry, Moscow, Russia

At carrying out the proteomic analysis of two isogenic Vibrio cholerae Dakka35 of the classical biovar it was revealed, that toxigenic (1 type) and nontoxigenic (2 type) clones differ from each other not only the expression ofgenes of exopolysaccharide, motility, and soluble haemagglutinin/protease, but also change of activity about other 60 genes. Among 11 identified proteins 5 are the enzymes participating in a metabolism cells. Besides it is revealed, that clones 2 types of Dakka35 strain synthesize in a more level of OmpU and TolC proteins, which provide their more significant stability to action of bile in comparison with clones of 1 type. It was shown, that bile serves as a signal from an environment for switching of gene expression of the genes, coding production of factors as virulence, and carrying out protective function of bacterial cell.
Zh.Mikrobiol. (Moscow), 2006, 3, P. 11—16