Branch for the Production of Bacterial Preparations of the Research and Production Amalgamation “Microgen”, Moscow, Russia
The development of large-scale biotechnological production with the use continuous cell-line cultures as producers revealed the role of apoptosis in the culture as a factor producing essential influence on the effectiveness of the process, the level of the accumulation of the target product and its quality. The limiting influence of apoptosis as the programmed death of cells in the culture was determined by a number of factors: the chemical composition, pH and chemical properties of the medium; the levels of glucose, amino-acid and vitamin depletion; the uniformity of oxygenation; temperature conditions; the mode of agitation and the intensity of hydrodynamic forces; the content of mitogenetic factors and toxins in the medium; the presence and activity of the expression of apoptosis stimulators and inhibitors. The presence of correlation between the intensity of apoptosis, cultural properties and the characteristics of the mitotic cycle of the cell line was shown. The latter made it possible to regard the biological properties of the producer culture as one of the leading factors modifying the process of the programmed death of cells.
Zh. Mikrobiol. (Moscow), 2005, No. 6, P. 99—105