Akhmatova, N.K., Lebedinskaya, O.V., Kiselevsky, M.V., Makashin, A.I., Semenova, I.B., Semenov, B.F.

Generation of dendrite cells and the use of immunomodulators of bacterial origin as ripening inducers

Mechnikov Research Institute for Vaccines and Sera, Moscow; State Medical Academy, Perm; Blokhin Oncological Research Center, Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia

The generation of ripe dendrite cells (DC) of marrow origin was obtained with the use of the vaccine Immunovac-BП-4, an immunomodulator of microbial origin, as well as Klebsiella pneumoniae LPS and TNF-α, as ripening inducers. These inducers equally led to the ripening of DC. The generation of ripe DC was characterized by morphological, phenotypical and functional changes. The immunophenotype of cells altered from CD34⁺, CD38‾, CD40‾, CD80‾, CD86‾, MHC I‾, MHC II‾, F4/80‾ to CD34‾, CD38⁺, CD40⁺, CD80⁺, MHC I⁺, MHC II⁺, F4/80low. In parallel with the ripening of DC their phagocytic activity decreased. In culture medium with ripe DC the levels of such cytokines as IL-1b, IL-6, IL-12, IFN-γ, TNF-α significantly increased and the production of IL-4 decreased. The content of IL-2 and IL-10 remained unchanged.
Zh. Mikrobiol. (Moscow), 2005, No. 5, P. 57—62