Military Medical Academy, St. Petersburg, Russia
The profiles of the utilization of 20 protein amino acids in 118 Klebsiella pneumoniae subsp. pneumoniae, K.oxytoca, K.planticola, K.mobilis, Enterobacter cloacae, Serratia marscescens, S.liquefaciens, Escherichia coli strains isolated from clinical material were studied. The utilization of amino acids was determined on minimal sa-line agar containing amino acid as the only source of nitrogen and carbon; the results were evaluated after 72-hour incubation at 37°C. 17 profiles of amino-acid utilization were thus determined, most of them genus-specific in enterobacteria: Klebsiella (profiles No. 1 — 6, 9, 10), Enterobacter (No. 11 — 13), Serratia (No. 14 — 16), Es-cherichia (No. 17). The full coincidence of amino-acid utilization profiles in bacteria of K.mobilis (No. 1, 6) and K.pneumoniae subsp. pneumoniae with out of such profiles in bacteria of the genera Enterobacter, Serratia, Es-cherichia was established, which confirmed that K.mobilis (formerly Enterobacter aerogenes) belonged to the genus Klebsiella.
Zh. Mikrobiol. (Moscow), 2005, No. 5, P. 11—14