Orlova, O.E., Elkina, S.I., Yastrebova, N.E., Vaneeva, N.P., Sergeev, V.V., Kalina, N.G., Tokarskaya, M.M.

Influence of the aminopeptide concentration on the growth of Haemophilus influenzae, type b, and the synthesis of its capsular polysaccharide

Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia

The influence of the aminopeptide concentration on the growth of H.influenzae b culture and the synthesis of H.influenzae b capsular polysaccharide was determined. The maximum amount of capsular polysaccharide was accumulated at the concentration of aminopeptide in the culture fluid reaching 50 ml/l. An increase in the aminopeptide concentration led to a decreased amount of synthesized polysaccharide and an increased amount of biomass. The decrease of the aminopeptide concentration to 10 ml/l resulted in decreased amounts of both biomass and synthesized polysaccharide.
Zh. Mikrobiol.(Moscow), 2005, No. 3, P. 92—95