Kuban State Medical Academy, Krasnodar, Russia
The combined use of laser radiation and Radachlorine™ revealed the presence of correlation between the number of viable Streptococcus pyogenes cells and the concentration of photosensitizer (r=-0.62), time of irradiation (r=–0.75), as well as energy density (r=-0.80). The death of 100% of suspended S.pyogenes cells was observed at the concentration of Radachlorine™ reaching 14.4 μM and radiation power reaching 1 W, irrespective of energy density. Pulse and superpulse laser radiation, carried out simultaneously with the use of Radachlorine™ at a concentration of 1.8 mM, resulted in the death of 100% of S.pyogenes cells in vitro. On the experimental model of inflammation the application of 0.1% Radachlorine™ gel demonstrated higher effectiveness (in comparison with 0.35% Radachlorine™ solution) when used simultaneously with laser radiation, both continuous and superpulse (with energy density being 400 j/cm2 and power being 1 W). The use of these parameters in the treatment of the experimental foci of inflammation in the animals led to the complete elimination of S.pyogenes from infected tissues.
Zh. Mikrobiol. (Moscow), 2005, No. 3, P. 22—27