Scientific-production association “Immunopreparation, Ufa, Bashkortostan; Mechnikov Research Institute for Vaccines and Sera, Moscow, Russia
During the cultivation of B.subtilis strain 3H under optimum conditions (adequate nutrient medium, seed culture, temperature, the level of dissolved oxygen) protease was produced. Protease could be obtained in the purified form by means of gel chromatography and ultrafiltration. The isolated protease was immobilized on polyglucin and stabilized by intramolecular cross-linking with the use of glutaraldehyde. The comparison of native protease modified with polyglucin and glutaraldehyde, as well as with polyglucin, revealed advantages in the stability of the latter.
Zh. Mikrobiol. (Moscow), 2005, No. 3, P. 15—18