Zakharova, N.E., Sukhanova, S.M., Belyaeva, G.A.

Comparative characterization of laboratory-made selective nutrient media for Salmonella accumulation

Tarasevich State Research Institute for Standardization and Control of Medical Biological Preparations, Moscow, Russia

In this work the results of the trial of three selective nutrient media for Salmonella accumulation, viz. Leifson’s selenite broth produced by the Research and Manufacturing Amalgamation (RMA) “Nutrient Media”, tetrarathionate broth (Mǖller’s medium), laboratory-prepared, and newly developed MA-broth produced by the RMA “Nutrient Media”, are presented. The results of 6- and 24-hour incubation in these selective media was evaluated. As found in this study, the laboratory-prepared medium was most effective for the accumulation of salmonellae (S.paratyphi, S.typhi, S.gallinarum). With respect to the possible concomitant microflora (Escherichia coli and shigellae), the inhibiting properties of MA-broth were superior to those of Leifson’s medium, but inferior to those of Mǖller’s medium.
Zh. Mikrobiol. (Moscow), 2004, No. 5, P. 101—103