Ermolenko, E.I., Isakov, V.A., Zhdan-Pushkina, S.Kh., Tets, V.V.

Quantitative characterization of antagonistic activity of lactobacilli

Pasteur Research Institute of Epidemiology and Microbiology, Pavlov State Medical University, St. Petersburg, Russia

In this work the method of serial dilutions of lactobacilli in two-layer agar was used. On the agar surface bacterial or fungal cultures were applied at different time intervals. A special quantitative characteristic was introduced. L.plantarum strain 8P-À3 was shown to have the maximum antagonistic activity. In great amounts L.casei and L.reuteri are capable to suppress the growth of bacteria and fungi. All lactobacilli under study produced a pronounced bactericidal effect on Pseudomonas, had different influence on the viability of Escherichia and staphylococci and exhibited fungistatic and fungicidal action only when inoculated at high concentrations.
Zh. Mikrobiol. (Moscow), 2004, No. 5, P. 94—98