Institute of Molecular Biology — State Research Center of Virology and Biotechnology “Vector”, Koltsovo, Novosibirsk region; Regional Center for Prophylaxis and Control of AIDS, Novosibirsk, Russia
The nucleotide sequence of the variant of human immunodeficiency virus of type 1 (HIV-1), mostly widespread on the territory of the Novosibirsk region, was determined. The analysis of the nucleotide sequence confirmed that this variant belonged to HIV-1 of subtype A. The HIV-1 recombinant variant of subtype envB/envA with the recombination area within the second conservative region C2 of gene env, so far unknown, was detected and characterized. In HIV-1 the area at the begi
ing of gene env (5’-env) was found to belong to subtype B and the sequence at the end of gene env (3’-env), to subtype A. The analysis of the amino acid sequence of the third variable region of gene env demonstrated that the viruses under study belonged to macrophagotropic “slow/low” variants, characterized by low replication speed. The analysis of nucleotide sequences of the isolated variants of HIV-1 revealed their close genetic relationship with HIV-1 isolates circulating on the territory of Ukraine.
Zh. Mikrobiol. (Moscow), 2004, No. 5, P. 53—58