Research Institute of Agriculture, Penza, Russia; Republican Oncological Center, Tashkent, Republic of Uzbekista
nA nutrient medium for the cultivation of C.diphtheriae toxigenic strain was developed on the basis of raw materials unsuitable for use as foodstuffs and its main physico-chemical and cultivation properties were studied. The morphological, cultural, biochemical and toxigenic properties of C.diphtheriae cultivated in the experimental medium with horse serum were evaluated. As revealed in this study, C.diphtheriae retained their properties after prolonged cultivation and storage in the newly developed medium, both liquid and with agar added. The medium has a number of advantages: it is economical, raw materials for its production are readily available, the medium is free of ballast substances.
Zh. Mikrobiol. (Moscow), 2004, No. 4, P. 76—78