Timchenko, N.F., Terentyev, L.L., Nedashkovskaya, E.P., Terentyeva, N.A., Raznik, N.V., Pridnya, O.V., Rasskazov, V.A.

In vitro action of Yersinia pseudotuberculosis heat stable lethal toxin on the biosynthesis of protein

Research Institute of Epidemiology and Microbiology, Pacific Institute of Bioorganic Chemistry, Vladivostok, Russia

The results of the in vitro action of Y.pseudotuberculosis heat stable lethal toxin on the biosynthesis of protein are presented. The toxin was shown to inhibit the inclusion of exogenous amino acid into newly synthesized peptides. The degree of the inhibition of the biosynthesis of protein in the in vitro system depends on the amount of the toxin added to the incubation mixture. The use of the conjugated transcription and translation system confirms our earlier data on the influence of the lethal toxin on the biosynthesis of protein in eukaryotic cells in vivo.
Zh. Mikrobiol. (Moscow), 2004, No. 3, P. 15—18