State Research Center of Virology and Biotechnology “Vector”, Koltsovo, Novosibirsk region, Russia
A new promising method for obtaining some variants of effective new generation synthetic vaccines was developed. These immunogens were high-polymer antigenic structures in the form of virus-like particles with the polynucleotide complex (two-spiral RNA as the stimulator of nonspecific resistance) in the center and hybrid proteins, containing the epitopes of the infectious agent and the enzyme, on the surface. The central nucleus and the proteins were linked by the anion conjugate: spermidine-polyglucin substrate for the enzyme. A variant of the candidate vaccine (containing hybrid protein gp41(607-620)-β-galactosidase) against HIV-1 was obtained and its properties were studied. As shown in the immunization tests on laboratory animals (mice), the particles induced a higher titer of antibodies and prolonged immune response, acting as the depot of the antigens of the infective agent.
Zh. Mikrobiol. (Moscow), 2003, No. 6, P. 41—45