State Agricultural University; State University, Saratov, Russia
Aim. To study effect of lectin L II of Paenibacillus polymyxa 1460 on cytokine status of mice during modeling of infections caused by Staphylococcus, Escherichia and Yersinia. Materials and methods. Lectin LII of P.polymyxa 1460, bacterial cultures Staphylococcus aureus 209-P, Escherichia coli O1, and Yersinia enterocolitica 12 were used. Mice were inoculated intraperitoneally with 0.2 ml of suspension of bacterial culture grown during 24 hours (5,000 m.c./ml). Lectin (0.4 mcg/ml) in dose 0.2 ml was administered 24 h before and 1 h after inoculation. Serum samples from sacrificed animals were obtained 1, 6, and 24 hours after inoculation and concentrations of IL-1, IL-6, and TNF-α were measured in them using optical density values. Results. It was established that level of TNF-α in serum decreased during staphylococcal infection, whereas levels of IL-1 and IL-6 decreased during all modeled infections. Ability for correction of cytokine balance in organism of experimental animals by administration of lectin 24 h before and 1 h after inoculation with bacteria was demonstrated. Conclusion. Presented studies testify to the effect of bacterial lectin on cytokine-production activity of macrophages during phagocytosis of bacteria and infectious process.
Zh. Mikrobiol. (Moscow), 2009, No. 5, P. 82—85