Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia
Aim. Until now, the problem of effective therapy of HIV-infection is not resolved due to integrative type of interaction of HIV virus with target cell — T-lymphocyte. The study was aimed on search of method of deletion of HIV DNA-provirus from cell"s genome. Materials and methods. Non-pathogenic for humans Mycoplasma arginini was used for co-infection of HIV-infected cells in model systems in vitro. Results. Complex of mechanisms was documented leading to: blocking up to 50 — 60% of extracellular virus (according to titration results), cancel of apoptosis in infected cells stained on Hoechst, formation of defective vif- virions, which together with arginine-desaminase of M.arginini arrange permissive conditions for activation of cellular APOBEC3G with subsequent disruption of DNA-provirus and blocking of viral infection. As studies of ultrastructure showed, listed events resulted from direct interaction of HIV with mycoplasma. Conclusion. The elimination of HIV DNA-provirus is possible by co-infection of T-lymphocytes with M.arginini.
Zh. Mikrobiol. (Moscow), 2009, No. 4, P. 49—53