Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia
Aim. To study molecular and cellular mechanisms of action of "Staphylovac" vaccine on effectors of innate immunity in mice. Materials and methods. Dynamics of changes of mice spleen mononuclear leukocytes immunophenotype under the influence of the vaccine at determined time intervals was assessed in the study. Results. In response to antigenic stimulation mice immune system reacted by increasing number of cells expressing molecules of T-cell subpopulations — CD3, CD4, CD8; B-cell subpopulation — CD19 and natural killers — NK. Activation of immune system of mice was noted which expressed as increased levels of molecules for antigenic presentation (MHCI, MHCII), regulatory molecules (CD4/CD25) as well as enhanced cytotoxicity potential and phagocytic activity. Conclusion. Staphylococcal vaccine as well as studied antigens of Staphylococcus aureus promote activation of immune system. Cytotoxic potential obtained by NK cells under their influence as well as enhancement of phagocytic activity of macrophages point to activation of innate immune mechanisms.
Zh. Mikrobiol. (Moscow), 2009, No. 3, P. 32—37