Shemyakin,I.G., Stepanshina,V.N., Korobova,O.V., Anisimova,V.A., Ivanov,I.Yu., Lipin,M.Yu., Lazarev,A.A., Skaldina,A.I., Chernavskaya,L.A., Tarasova,T.I.

Genetic typing of Mycobacterium tuberculosis strains by spoligotyping and genome fingerprinting techniques

State Research Center of Applied Microbiology, Obolensk, Moscow region; Regional Tuberculosis Dispensary, Tula; TMO “Phthisiatry”, Kaluga, Russia

A total of 122 M.tuberculosis clinical drug-resistant strains isolated in Central Russia were studied by spoligotyping and genome fingerprinting techniques. According to spoligotyping results 77% of M.tuberculosis strains were distributed to 13 oligotypes, while 23% of these strains were found to form unique patterns. Most of them belonged to the families Beijing and Haarlem (43.4% and 13.9% respectively). The patterns of the strains of oligotype 12 (7F-7F-7E-0E-78-3E) were identical to those of the strains isolated in Brazil, France and the Netherlands. The strains of the spoligotype 22 (7F-1E-7F-7F-07-3E) had the patterns identical to those of the strains of group S13, also isolated in Brazil. According to genome fingerprinting 31.4% of the strains were found to belong to clusters with the similarity coeffecient equal to 1. The strains belonging to genotypes W and A1 were found to prevail in the analyzed group.
Zh. Mikrobiol. (Noscow), 2002, No. 6, P. 30—35