Merkulov, V.A., Lebedev, V.N., Plekhanova, T.M., Maksimov, V.A., Korovkin, S.A., Mironov, A.N.

Using reverse genetics method for developing recombinant strains of influenza viruses acceptable for use as live attenuated vaccines

Branch 48 of Central Research Institute of Ministry of Defense of Russian Federation — Virological Center, Sergiev Posad; Scientific-Manufacturing Organization «Microgen», Moscow, Russia

Perspectives of using reverse genetics methods for constructing of recombinant influenza virus strains acceptable for use as live attenuated vaccines are discussed. Using of attenuated NS-vectors of influenza virus opens possibilities for the development of recombinant vaccines with optimal ratio of immunogenicity and safety. Reverse genetics is applicable for development of effective vaccines against new pathogens such as highly pathogenic avian influenza A/H5N1.
Zh. Mikrobiol. (Moscow), 2009, No. 2, P. 111—117