Research Institute of Medical Primatology, Sochi-Adler, Russia
Aim. To study the role of neutralizing antibodies (NA) on the model of SHIV-infection in pig-tailed macaques immunized by different methods. Materials and methods. Titers of NA were determined using TZM-bl cells; titers corresponding to 50% neutralization were determined from serum dilution — relative luminescence graph. We have proposed to use index of integral neutralizing activity, which mathematically expressed as integral of neutralization function in serum dilutions interval from 1 (or first dilution used) to that giving selected titer (we selected 50% neutralization). Results. On the basis of analysis of data on course of SHIV-infection in previously immunized macaques it was concluded that more clear correlation exists between results of infection and integral neutralizing activity of serum compared with NA titers corresponding to 50% neutralization. Conclusion. Proposed method could be useful for analysis of immunization efficacy against HIV infection.
Zh. Mikrobiol. (Moscow), 2009, No. 2, P. 68—70