Gankovskaya, O.V.,Bakhareva, I.V., Gankovskaya, L.V., Somova, O.Yu., Zverev, V.V.

Study of expression of TLR9, NF-κB, TNFα genes in cells of cervical canal mucosa in pregnant women with herpesvirus infection

Mechnikov Research Institute of Vaccines and Sera; Russian State Medical University, Moscow, Russia

Aim. To study changes in expression of TLR9, NF-κB, TNFα genes in cervical canal mucosa in pregn­ant women with herpesvirus infection. Materials and methods. Using previously developed real-time polymerase chain reaction assays, levels of expression of genes, which corresponding proteins play important role in TLR9-dependent signaling pathway, was quantified. Results. Two-fold, 4.4-fold, and 3-fold rise in expression of TLR9, NF-κB, and TNFα genes respectively was revealed in pregnant women with herpesvirus infection compared to women with physiologically occurring pregnancy. Conclusion. Obtained results demonstrated the activation of TLR-mediated mechanisms of innate immunity on the level of cervical canal mucosa during pregnancy complicated by viral infection.
Zh. Mikrobiol. (Moscow), 2009, No. 2, P. 61—64