48 Central Research Institute of Ministry of Defense of Russian Federation, Kirov; Institute of Physiology, Syktyvkar, Russia
Aim. To study relationship between Yersinia pseudotuberculosis cultivation temperature and immunobiological properties of lypopolysaccharide (LPS). Materials and methods. LPS producing strain — Yersinia pseudotuberculosis 164/84 OX; experimental animal — guinea pigs, rabbits; methods — immunochemical (indirect hemagglutionation assay, RDP), physico-chemical (electrophoresis, gas-liquid chromatography), standard biochemical and statistical methods. Results. It was established that increase of Yersinia pseudotuberculosis cultivation temperature from 10°C to 37°C was associated with attenuation of LPS toxicity in guinea pigs, decrease of its immunochemical activity and contents and degree of acylation of 3OH-14:0 hydoxyacid as well as amount of electrophoretically detected O-side chains. It was assumed that the polysaccharide component plays its role in LPS toxicity. Conclusion. Relation between Yersinia pseudotuberculosis cultivation temperature and structural and functional properties of LPS preparations obtained from the culture was determined.
Zh. Mikrobiol. (Moscow), 2009, No. 1, P. 7—11