Poveshchenko,A.F., Perminova,O.M., Dushkin,M.I., Kuznetsov,P.O., Volsky,N.N., Kozlov,V.A.

Influence of modified low-density lipoproteins on humoral immune response and functional activity of macrophages in mice

Institute of Clinical Immunology, Institute of Therapy (Siberian Branch of the Russian Acad. Med. Sci.), Novosibirsk, Russia

Intravenous injection of acetylated low density lipoproteins (acLDL) in mice in a dose of 0.5 mg per mouse decreased the intensity of humoral immune response to sheep red blood cells (SRBC) by 35%. The addition of acLDL to mouse peritoneal macrophages in vitro resulted in inhibition of Fc-dependent phagocytosis of SRBC and fourfold increased secretion of prostaglandins E₂ by macrophages. Fc-dependent phagocytosis of SRBC was also found to be inhibited by oxysterols (25-hydroxycholesterol and 7-ketocholesterol), added to the incubation medium of macrophages in vitro in doses of 0.5 — 5 мg/ml. The conclusion was made that oxidative metabolism of cholesterol and arachidonic acid, contained in LDL, may mediate the immunomodulating effects of modified LDL.
Zh. Mikrobiol. (Moscow), 2002, No. 1, P. 57—60