Gatypova, E.V., Zlygostev, S.A., Kaloshin, A.A., Mikhaylova, N.A.

Obtaining of outer membrane protein I of Pseudomonas aeruginosa and assessment of its antigenic properties

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

Gene of outer membrane protein I (OprI) of Pseudomonas aeruginosa was cloned in Escheri­chia coli cells. Synthesized protein OprI contained additional sequence of 6 histidines on the N-terminus, which allowed its chromatographic purification in Ni-agarose. Obtained recombinant ptotein specifically reacted with hyperimmune rabbit serum against whole-cell P. aeruginosa and stimulated synthesis of specific antibodies in immunized mice and rabbits. Obtained hyperimmune rabbit sera against recombinant protein OprI had directive antimicrobial activity against P.aeruginosa.
Zh. Mikrobiol. (Moscow), 2008, No. 6, P. 50—53