Kuban State Medical University, Krasnodar, Russia
Aim. To study indicators of immune status (leukocytes, CD3+-, CD4+-, CD8+-, CD16+-, CD20+-lymphocytes counts, IgA, IgM, IgG, IL-2, circulating immune complexes levels, nitroblue tetrazolium test) in 30 HIV-infected patients with disseminated tuberculosis compared with morphologic features of tuberculosis process in lymphatic nodes at different stages of HIV-infection (11 autopsy observations). Materials and methods. The clonal antibodies method was used to differentiate immunocompetent cells by the qualitative antigen composition on cell membranes. The number of CD-cells was determined by flow cytometry. Results. Two stages of HIV-infection were established (early ― 2B, and late ― 4B-5) and histopathologic features of tuberculosis were related to stage of HIV-infection: at early stage of HIV-infection morphologic reactions of immune system were preserved (monocytoid reaction and lymphopenia were presented), whereas at later stages, according to autopsy data, disseminated and generalized tuberculosis on the background of severe immunodeficiency (hypoplasia and aplasia of lymphocytes) was predominated. Conclusion. Indicators of immune status in HIV-infected patients with disseminated tuberculosis depended from stage of HIV-infection (from early stages to late ones indicators of cellular immunity decreased with parallel increase of humoral immunity indicators). The degree of decrease of phagocytic function of neutrophils correlated with severity and activity of inflammation in patients with associated tuberculosis and HIV-infection.
Zh. Mikrobiol. (Moscow), 2008, No. 5, P. 101—103