Kaloshin, A.A., Zlygostev, S.A., Gatypova, E.V., Mikhailova, N.A.

Study of immunogenic properties of recombinant outer membrane proteins F and L from Pseudomonas aeruginosa

Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia

Recombinant outer membrane proteins OprF, OprL, and C-terminal part of OprF protein (OprF(192-342)) was synthesized in Escherichia coli M15 cells. It was shown that in rabbits immunized with chromatographically purified recombinant proteins mounted specific antibody response. In experiments in virto obtained hyperimmune to OprF, OprL, and OprF(192-342) rabbit sera inhibited growth of P.aeruginosa culture and in experiments in vivo protected mice from infection caused by this microorganism.
Zh. Mikrobiol. (Moscow), 2008, No. 5, P. 76—79