Antonov, V.A., Ilyukhin, V.I., Senina, T.V., Loboiko, A.D., Tkachenko, G.A., Zinchenko, O.V., Mazurova, I.Yu.

Phenotypic and genotypic identification of bacteria from the Burkholderia cepacia complex

Research Institute for Plague Control, Volgograd, Russia

Aim. Evaluation of the diagnostic value of pheno- and genotypic characteristics of B.cepacia strains collection. Materials and methods. Phenotypic and genetic methods of identification and differentiation of 25 strains of the B.cepacia complex. Results. Polyphasic taxonomic approach utilizing multiple diagnostic tests was used for accurate identification of Burkholderia species. Algorithm for identification of microorganisms from the B.cepacia complex was developed. Conclusion. Combined use of phenotypic and molecular genetic tests, such as recA gene PCR, is recommended for differentiation of the B.cepacia complex genomovars.
Zh. Mikrobiol. (Moscow), 2008, No. 4, P. 78—82