Semenova, I.B., Vorobyev, D.S., Sveshnikov, P.G., Kurbatova, E.A.

Complex of recombinant heat shock protein with lypopolysaccharide induces rapid protection of mice against Salmonella typhimurium and avirulent for humans avian influenza virus H5N2

Gamaleya Research Institute of Epidemiology and Microbiology, Mechnikov Research Institute of Vaccines and Sera, Russian Research Center of Molecular Diagnostics and Therapy, Moscow, Russia

Protective effect of immunization with heat shock protein (HSP) against bacterial and viral infections in mice was studied. Recombinant HSP 70 kDa of Mycobacterium tuberculosis contaminated with lypopolysaccharide (0.185 mcg/ml) was used for experiments. One intraperitoneal injection of 100 or 400 mcg of HSP induced rapid protection against intraperitoneal challenge with 125 LD₅₀ of Salmonella typhimurium (on 3^rd — 6^th day) and against intranasal challenge with 10 LD50 of avirulent for humans avian influenza virus H5N2 (A/mallard/Pennsylvania/10218/84) (on 5^th — 8^th day). Three daily injections with 10 mcg of HSP induced rapid, significant and long-term protection against S.typhimurium. Immunization with HSP protected 100% of mice dur-ing 3 days after the challenge, 50% of immunized animals survived during 21 days (duration of the study). All non-immunized mice died on 6^th day.
Zh. Mikrobiol. (Moscow), 2007, No. 6, P. 54—57